The intratracheal instillation technique is used to deliver a variety of agents to the lungs
ranging from pathogens (bacteria, viruses), toxins, to therapeutic agents. To model lung
inflammation and injury, LPS can be administrated via intranasal, intratracheal, or aerosol
approaches. Each technique has its limitations. The intratracheal technique can involve the
non-invasive instillation method (via the oro-tracheal route) or a direct injection into the
trachea. Here, we describe an optimized method for direct visual instillation of LPS via the …