[HTML][HTML] Casein kinase 1 regulates connexin-43 gap junction assembly
CD Cooper, PD Lampe - Journal of Biological Chemistry, 2002 - ASBMB
Phosphorylation of members of the connexin family of gap junction proteins has been
correlated with gap junction assembly, but the mechanisms involved remain unclear. We
have examined the role of casein kinase 1 (CK1) in connexin-43 (Cx43) gap junction
assembly. Cellular co-immunoprecipitation experiments and in vitro CK1 phosphorylation
reactions indicate that CK1 interacted with and phosphorylated Cx43, initially on serine (s)
325, 328, or 330. 32 P i-Metabolically labeled cells treated with CKI-7, a specific CK1 …
correlated with gap junction assembly, but the mechanisms involved remain unclear. We
have examined the role of casein kinase 1 (CK1) in connexin-43 (Cx43) gap junction
assembly. Cellular co-immunoprecipitation experiments and in vitro CK1 phosphorylation
reactions indicate that CK1 interacted with and phosphorylated Cx43, initially on serine (s)
325, 328, or 330. 32 P i-Metabolically labeled cells treated with CKI-7, a specific CK1 …