Quantitation of free and total N-acetylcysteine amide and its metabolite N-acetylcysteine in human plasma using derivatization and electrospray LC-MS/MS
B King, J Vance, GM Wall, R Shoup - Journal of Chromatography B, 2019 - Elsevier
Studies of N-acetylcysteine amide (NACA) in nonclinical models have demonstrated various
antioxidant, anti-apoptotic, anti-inflammatory and neuroprotective effects, and it is currently
being developed as a treatment for retinitis pigmentosa. Sensitive LC-MS/MS methods were
developed and validated to quantitate reduced and total NACA and its major metabolite, N-
acetylcysteine (NAC), in human plasma to support clinical studies involving NACA. To trap
and stabilize reduced NACA and NAC at the time of collection, whole blood was …
antioxidant, anti-apoptotic, anti-inflammatory and neuroprotective effects, and it is currently
being developed as a treatment for retinitis pigmentosa. Sensitive LC-MS/MS methods were
developed and validated to quantitate reduced and total NACA and its major metabolite, N-
acetylcysteine (NAC), in human plasma to support clinical studies involving NACA. To trap
and stabilize reduced NACA and NAC at the time of collection, whole blood was …