[HTML][HTML] Heparan sulfate and heparin promote faithful prion replication in vitro by binding to normal and abnormal prion proteins in protein misfolding cyclic …
M Imamura, N Tabeta, N Kato, Y Matsuura… - Journal of Biological …, 2016 - ASBMB
The precise mechanism underlying the conversion of normal prion protein (PrP C) into
abnormal prion protein (PrP Sc) remains unclear. Protein misfolding cyclic amplification
(PMCA), an in vitro technique used for amplifying PrP Sc, results in PrP Sc replication that
preserves the strain-specific characteristics of the input PrP Sc; thus, PMCA mimics the
process of in vivo PrP Sc replication. Previous work has demonstrated that in PMCA, nucleic
acids are critical for PrP Sc amplification, but little information has been reported on …
abnormal prion protein (PrP Sc) remains unclear. Protein misfolding cyclic amplification
(PMCA), an in vitro technique used for amplifying PrP Sc, results in PrP Sc replication that
preserves the strain-specific characteristics of the input PrP Sc; thus, PMCA mimics the
process of in vivo PrP Sc replication. Previous work has demonstrated that in PMCA, nucleic
acids are critical for PrP Sc amplification, but little information has been reported on …