Gene targeting restricted to mouse striated muscle lineage
P Miniou, D Tiziano, T Frugier, N Roblot… - Nucleic acids …, 1999 - academic.oup.com
P Miniou, D Tiziano, T Frugier, N Roblot, M Le Meur, J Melki
Nucleic acids research, 1999•academic.oup.comSpatially and temporally regulated somatic mutations can be achieved by using the
Cre/LoxP recombination system of bacteriophage P1. In order to develop gene knockouts
restricted to striated muscle, we generated a transgenic mouse line expressing Cre
recombinase under the control of the human α-skeletal actin promoter. Specific excision of a
loxP-flanked gene was demonstrated in striated muscle, heart and skeletal muscle, in a
pattern very similar to the expression of the endogenous α-skeletal actin gene. Therefore …
Cre/LoxP recombination system of bacteriophage P1. In order to develop gene knockouts
restricted to striated muscle, we generated a transgenic mouse line expressing Cre
recombinase under the control of the human α-skeletal actin promoter. Specific excision of a
loxP-flanked gene was demonstrated in striated muscle, heart and skeletal muscle, in a
pattern very similar to the expression of the endogenous α-skeletal actin gene. Therefore …
Abstract
Spatially and temporally regulated somatic mutations can be achieved by using the Cre/LoxP recombination system of bacteriophage P1. In order to develop gene knockouts restricted to striated muscle, we generated a transgenic mouse line expressing Cre recombinase under the control of the human α-skeletal actin promoter. Specific excision of a loxP-flanked gene was demonstrated in striated muscle, heart and skeletal muscle, in a pattern very similar to the expression of the endogenous α-skeletal actin gene. Therefore, the reported transgenic line can be used to target inactivation or activation of a given gene to the skeletal muscle lineage.
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