RrgA and RrgB Are Components of a Multisubunit Pilus Encoded by the Streptococcus pneumoniae rlrA Pathogenicity Islet
J LeMieux, DL Hava, A Basset, A Camilli - Infection and immunity, 2006 - Am Soc Microbiol
J LeMieux, DL Hava, A Basset, A Camilli
Infection and immunity, 2006•Am Soc MicrobiolThe rlrA pathogenicity islet in Streptococcus pneumoniae TIGR4 encodes three surface
proteins, RrgA, RrgB, and RrgC, and three sortase enzymes. Using transmission electron
microscopy, cell fractionation, cell wall sorting signal domain swapping, and Western
blotting, we show that RrgA and RrgB are incorporated into a multisubunit pilus in S.
pneumoniae.
proteins, RrgA, RrgB, and RrgC, and three sortase enzymes. Using transmission electron
microscopy, cell fractionation, cell wall sorting signal domain swapping, and Western
blotting, we show that RrgA and RrgB are incorporated into a multisubunit pilus in S.
pneumoniae.
Abstract
The rlrA pathogenicity islet in Streptococcus pneumoniae TIGR4 encodes three surface proteins, RrgA, RrgB, and RrgC, and three sortase enzymes. Using transmission electron microscopy, cell fractionation, cell wall sorting signal domain swapping, and Western blotting, we show that RrgA and RrgB are incorporated into a multisubunit pilus in S. pneumoniae.
American Society for Microbiology