Optimization and validation of the TZM-bl assay for standardized assessments of neutralizing antibodies against HIV-1
M Sarzotti-Kelsoe, RT Bailer, E Turk, C Lin… - Journal of …, 2014 - Elsevier
The TZM-bl assay measures antibody-mediated neutralization of HIV-1 as a function of
reductions in HIV-1 Tat-regulated firefly luciferase (Luc) reporter gene expression after a
single round of infection with Env-pseudotyped viruses. This assay has become the main
endpoint neutralization assay used for the assessment of pre-clinical and clinical trial
samples by a growing number of laboratories worldwide. Here we present the results of the
formal optimization and validation of the TZM-bl assay, performed in compliance with Good …
reductions in HIV-1 Tat-regulated firefly luciferase (Luc) reporter gene expression after a
single round of infection with Env-pseudotyped viruses. This assay has become the main
endpoint neutralization assay used for the assessment of pre-clinical and clinical trial
samples by a growing number of laboratories worldwide. Here we present the results of the
formal optimization and validation of the TZM-bl assay, performed in compliance with Good …