Establishing and characterizing a CD30‐positive cell line harboring HHV‐8 from a primary effusion lymphoma

H Katano, Y Hoshino, Y Morishita… - Journal of medical …, 1999 - Wiley Online Library
H Katano, Y Hoshino, Y Morishita, T Nakamura, H Satoh, A Iwamoto, B Herndier, S Mori
Journal of medical virology, 1999Wiley Online Library
Primary effusion lymphoma (PEL, or body‐cavity‐based lymphoma [BCBL]) is a new
subtype of non‐Hodgkin's lymphoma in which tumor cells locate in the body cavity
exclusively. PEL/BCBL is widely accepted as one of the neoplastic complications of AIDS,
associated mostly with human herpesvirus 8 (HHV‐8/Kaposi's sarcoma‐associated
herpesvirus [KSHV]) and Epstein‐Barr virus (EBV). We established and characterized a PEL
cell line named TY‐1 from a 47‐year‐old patient with AIDS. TY‐1 exhibits indeterminate …
Abstract
Primary effusion lymphoma (PEL, or body‐cavity‐based lymphoma [BCBL]) is a new subtype of non‐Hodgkin's lymphoma in which tumor cells locate in the body cavity exclusively. PEL/BCBL is widely accepted as one of the neoplastic complications of AIDS, associated mostly with human herpesvirus 8 (HHV‐8/Kaposi's sarcoma‐associated herpesvirus [KSHV]) and Epstein‐Barr virus (EBV). We established and characterized a PEL cell line named TY‐1 from a 47‐year‐old patient with AIDS. TY‐1 exhibits indeterminate immunophenotype, expressing CD45 and CD30 cell surface antigens but not expressing B‐ or T‐cell markers. Cytogenetic analysis revealed the representative karyotype of 50,XYq‐,+7,+8,+11,+15. Southern blot analysis demonstrated HHV‐8 and EBV genomes in the original tumor cells obtained from the pericardial effusion, while HHV‐8 but not EBV was detected in TY‐1 using PCR or Southern blot analysis. Tetradecanylphorbol acetate treatment induced some TY‐1 cells to proceed to the reproductive phase. This cell line may be an useful tool for research on PEL and HHV‐8. J. Med. Virol. 58:394–401, 1999. © 1999 Wiley‐Liss, Inc.
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