[HTML][HTML] Electroporation of mice zygotes with dual guide RNA/Cas9 complexes for simple and efficient cloning-free genome editing
M Teixeira, BF Py, C Bosc, D Laubreton, MJ Moutin… - Scientific reports, 2018 - nature.com
M Teixeira, BF Py, C Bosc, D Laubreton, MJ Moutin, J Marvel, F Flamant, S Markossian
Scientific reports, 2018•nature.comIn this report, we present an improved protocol for CRISPR/Cas9 genome editing in mice.
The procedure consists in the electroporation of intact mouse zygotes with ribonucleoprotein
complexes prepared in vitro from recombinant Cas9 nuclease and synthetic dual guide
RNA. This simple cloning-free method proves to be extremely efficient for the generation of
indels and small deletions by non-homologous end joining, and for the generation of
specific point mutations by homology-directed repair. The procedure, which avoids DNA …
The procedure consists in the electroporation of intact mouse zygotes with ribonucleoprotein
complexes prepared in vitro from recombinant Cas9 nuclease and synthetic dual guide
RNA. This simple cloning-free method proves to be extremely efficient for the generation of
indels and small deletions by non-homologous end joining, and for the generation of
specific point mutations by homology-directed repair. The procedure, which avoids DNA …
Abstract
In this report, we present an improved protocol for CRISPR/Cas9 genome editing in mice. The procedure consists in the electroporation of intact mouse zygotes with ribonucleoprotein complexes prepared in vitro from recombinant Cas9 nuclease and synthetic dual guide RNA. This simple cloning-free method proves to be extremely efficient for the generation of indels and small deletions by non-homologous end joining, and for the generation of specific point mutations by homology-directed repair. The procedure, which avoids DNA construction, in vitro transcription and oocyte microinjection, greatly simplifies genome editing in mice.
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