Isolation of normal and cancer-associated fibroblasts from fresh tissues by Fluorescence Activated Cell Sorting (FACS)
Y Sharon, L Alon, S Glanz, C Servais… - JoVE (Journal of Visualized …, 2013 - jove.com
Cancer-associated fibroblasts (CAFs) are the most prominent cell type within the tumor
stroma of many cancers, in particular breast carcinoma, and their prominent presence is
often associated with poor prognosis1, 2. CAFs are an activated subpopulation of stromal
fibroblasts, many of which express the myofibroblast marker α-SMA3. CAFs originate from
local tissue fibroblasts as well as from bone marrow-derived cells recruited into the
developing tumor and adopt a CAF phenotype under the influence of the tumor …
stroma of many cancers, in particular breast carcinoma, and their prominent presence is
often associated with poor prognosis1, 2. CAFs are an activated subpopulation of stromal
fibroblasts, many of which express the myofibroblast marker α-SMA3. CAFs originate from
local tissue fibroblasts as well as from bone marrow-derived cells recruited into the
developing tumor and adopt a CAF phenotype under the influence of the tumor …