Isolation of vascular smooth muscle cells from a single murine aorta

J Lynn Ray, R Leach, JM Herbert, M Benson - Methods in Cell Science, 2001 - Springer
J Lynn Ray, R Leach, JM Herbert, M Benson
Methods in Cell Science, 2001Springer
The vascular smooth muscle cell plays a significant role in many important cardiovascular
disorders, and smooth muscle biology is therefore important to cardiovascular research. The
mouse is critical to basic cardiovascular research, largely because techniques for genetic
manipulation are more fully developed in the mouse than in any othermammalian species.
We describe here a technique for isolating smooth muscle cells from a single mouse aorta.
This technique is particularly useful when material is limiting, as is frequently the case when …
Abstract
The vascular smooth muscle cell plays a significant role in many important cardiovascular disorders, and smooth muscle biology is therefore important to cardiovascular research. The mouse is critical to basic cardiovascular research, largely because techniques for genetic manipulation are more fully developed in the mouse than in any othermammalian species. We describe here a technique for isolating smooth muscle cells from a single mouse aorta. This technique is particularly useful when material is limiting, as is frequently the case when genetically modified animals are being characterized.
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