[PDF][PDF] Mitochondrial patch clamp of beige adipocytes reveals UCP1-positive and UCP1-negative cells both exhibiting futile creatine cycling

AM Bertholet, L Kazak, ET Chouchani… - Cell metabolism, 2017 - cell.com
AM Bertholet, L Kazak, ET Chouchani, MG Bogaczyńska, I Paranjpe, GL Wainwright…
Cell metabolism, 2017cell.com
Cold and other environmental factors induce" browning" of white fat depots—development of
beige adipocytes with morphological and functional resemblance to brown fat. Similar to
brown fat, beige adipocytes are assumed to express mitochondrial uncoupling protein 1
(UCP1) and are thermogenic due to the UCP1-mediated H+ leak across the inner
mitochondrial membrane. However, this assumption has never been tested directly. Herein
we patch clamped the inner mitochondrial membrane of beige and brown fat to provide a …
Summary
Cold and other environmental factors induce "browning" of white fat depots—development of beige adipocytes with morphological and functional resemblance to brown fat. Similar to brown fat, beige adipocytes are assumed to express mitochondrial uncoupling protein 1 (UCP1) and are thermogenic due to the UCP1-mediated H+ leak across the inner mitochondrial membrane. However, this assumption has never been tested directly. Herein we patch clamped the inner mitochondrial membrane of beige and brown fat to provide a direct comparison of their thermogenic H+ leak (IH). All inguinal beige adipocytes had robust UCP1-dependent IH comparable to brown fat, but it was about three times less sensitive to purine nucleotide inhibition. Strikingly, only ∼15% of epididymal beige adipocytes had IH, while in the rest UCP1-dependent IH was undetectable. Despite the absence of UCP1 in the majority of epididymal beige adipocytes, these cells employ prominent creatine cycling as a UCP1-independent thermogenic mechanism.
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