[HTML][HTML] Influenza-specific antibody-dependent phagocytosis
F Ana-Sosa-Batiz, H Vanderven, S Jegaskanda… - PLoS …, 2016 - journals.plos.org
PLoS One, 2016•journals.plos.org
Background Immunity to human influenza A virus (IAV) infection is only partially understood.
Broadly non-neutralizing antibodies may assist in reducing disease but have not been well
characterized. Methods We measured internalization of opsonized, influenza protein-coated
fluorescent beads and live IAV into a monocytic cell line to study antibody-dependent
phagocytosis (ADP) against multiple influenza hemagglutinin (HA) subtypes. We analyzed
influenza HA-specific ADP in healthy human donors, in preparations of intravenous …
Broadly non-neutralizing antibodies may assist in reducing disease but have not been well
characterized. Methods We measured internalization of opsonized, influenza protein-coated
fluorescent beads and live IAV into a monocytic cell line to study antibody-dependent
phagocytosis (ADP) against multiple influenza hemagglutinin (HA) subtypes. We analyzed
influenza HA-specific ADP in healthy human donors, in preparations of intravenous …
Background
Immunity to human influenza A virus (IAV) infection is only partially understood. Broadly non-neutralizing antibodies may assist in reducing disease but have not been well characterized.
Methods
We measured internalization of opsonized, influenza protein-coated fluorescent beads and live IAV into a monocytic cell line to study antibody-dependent phagocytosis (ADP) against multiple influenza hemagglutinin (HA) subtypes. We analyzed influenza HA-specific ADP in healthy human donors, in preparations of intravenous immunoglobulin (IVIG), and following IAV infection of humans and macaques.
Results
We found that both sera from healthy adults and IVIG preparations had broad ADP to multiple seasonal HA proteins and weak cross-reactive ADP to non-circulating HA proteins. The ADP in experimentally influenza-infected macaque plasma and naturally influenza-infected human sera mediated phagocytosis of both homologous and heterologous IAVs. Further, the IAV phagocytosed in an antibody-mediated manner had reduced infectivity in vitro.
Conclusion
We conclude that IAV infections in humans and macaques leads to the development of influenza-specific ADP that can clear IAV infection in vitro. Repeated exposure of humans to multiple IAV infections likely leads to the development of ADP that is cross-reactive to strains not previously encountered. Further analyses of the protective capacity of broadly reactive influenza-specific ADP is warranted.
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