[HTML][HTML] Dimeric galectin-1 induces surface exposure of phosphatidylserine and phagocytic recognition of leukocytes without inducing apoptosis
M Dias-Baruffi, H Zhu, M Cho, S Karmakar… - Journal of Biological …, 2003 - ASBMB
We report that human galectin-1 (dGal-1), a small dimeric β-galactoside-binding protein,
induces phosphatidylserine (PS) exposure, measured by Annexin V staining, on human
promyelocytic HL-60 cells, T leukemic MOLT-4 cells, and fMet-Leu-Phe-activated, but not
resting, human neutrophils. This effect of dGal-1 on HL-60 and MOLT-4 cells is enhanced by
pretreatment of the cells with neuraminidase, but treatment of resting neutrophils with
neuraminidase does not enhance their sensitivity to dGal-1. Although the induction of …
induces phosphatidylserine (PS) exposure, measured by Annexin V staining, on human
promyelocytic HL-60 cells, T leukemic MOLT-4 cells, and fMet-Leu-Phe-activated, but not
resting, human neutrophils. This effect of dGal-1 on HL-60 and MOLT-4 cells is enhanced by
pretreatment of the cells with neuraminidase, but treatment of resting neutrophils with
neuraminidase does not enhance their sensitivity to dGal-1. Although the induction of …