Generating Conditional Mutants to Analyze Ciliary Functions: The Use of Cre–Lox Technology to Disrupt Cilia in Specific Organs
AK O'Connor, RA Kesterson, BK Yoder - Methods in cell biology, 2009 - Elsevier
The list of human disordered associated with cilia dysfunction, the ciliopathies, continues to
highlight the importance of understanding the many roles of the long overlooked primary
cilium. Much of the insights into the clinical importance of the cilium have come from
analyses in model organisms, especially the mouse. However, the early embryonic lethality
and severe developmental defects associated with cilia disruption has hindered progress in
exploring cilia functions in late development or in adult tissues. This hurdle is being …
highlight the importance of understanding the many roles of the long overlooked primary
cilium. Much of the insights into the clinical importance of the cilium have come from
analyses in model organisms, especially the mouse. However, the early embryonic lethality
and severe developmental defects associated with cilia disruption has hindered progress in
exploring cilia functions in late development or in adult tissues. This hurdle is being …