In acute kidney injury, which is a significant cause of morbidity and mortality, cytokines and leukocytes promote inflammation and injury. We examined the pathogenic role of IL-17A in cisplatin-induced acute kidney injury. Intrarenal IL-17A mRNA transcription and protein expression were increased in wild-type mice after cisplatin-induced renal injury. An important role for IL-17A in the nephrotoxicity of cisplatin was demonstrated by observing protection from cisplatin-induced functional and histological renal injury in Il17a^−/− and Rorγt^−/− mice, as well as in mice treated pre-emptively with anti–IL-17A antibodies. Both renal injury and renal IL-1β and IL-17A production were attenuated in Asc^−/− and Tlr2^−/− mice, suggesting that cisplatin induces endogenous TLR2 ligand production and activates the ASC-dependent inflammasome complex, resulting in IL-1β and injurious IL-17A production. Neutrophils and natural killer cells are the likely targets of these pathways, because combined depletion of these cells was strongly protective; anti–IL-17A antibodies had no additional effect in this setting. Although IL-17A can also be produced by CD4⁺ and γδ T cells, IL-17A from those cells does not contribute to renal injury. Cisplatin-induced injury was unchanged in γδ T-cell–deficient mice, whereas Il17a^−/− CD4⁺ T cells induced similar injury as did wild-type CD4⁺ T cells on transfer to cisplatin-injected Rag1^−/− mice. These studies demonstrate an important role for TLR2, the ASC inflammasome, and IL-17A in innate leukocytes in cisplatin-induced renal injury.