DNA damage response (DDR) activates a complex signaling network that triggers DNA
repair, cell cycle arrest, and/or cell death. Depending on the type and severity of DNA lesion,
DDR is controlled by" master" regulators including ATM and ATR protein kinases. Cisplatin,
a major chemotherapy drug that cross-links DNA, induces ATR-dependent DDR, resulting in
apoptosis. However, it is unclear how ATR is activated. To identify the key regulators of ATR,
we analyzed the proteins that associate with ATR after cisplatin treatment by blue native …