[HTML][HTML] Identification of intron and exon sequences involved in alternative splicing of insulin receptor pre-mRNA
A Kosaki, J Nelson, NJG Webster - Journal of Biological Chemistry, 1998 - ASBMB
The insulin receptor exists as two isoforms, A and B, that result from alternative splicing of
exon 11 in the primary transcript. We have shown previously that the alternative splicing is
developmentally and hormonally regulated. Consequently, these studies were instigated to
identify sequences within the primary RNA transcript that regulate the alternative splicing.
Minigenes containing exons 10, 11, and 12 and the intervening introns were constructed
and transfected into HepG2 cells, which contain both isoforms of the insulin receptor. The …
exon 11 in the primary transcript. We have shown previously that the alternative splicing is
developmentally and hormonally regulated. Consequently, these studies were instigated to
identify sequences within the primary RNA transcript that regulate the alternative splicing.
Minigenes containing exons 10, 11, and 12 and the intervening introns were constructed
and transfected into HepG2 cells, which contain both isoforms of the insulin receptor. The …