Photothrombotic ischemia: a minimally invasive and reproducible photochemical cortical lesion model for mouse stroke studies

V Labat-Gest, S Tomasi - JoVE (Journal of Visualized Experiments), 2013 - jove.com
The photothrombotic stroke model aims to induce an ischemic damage within a given
cortical area by means of photo-activation of a previously injected light-sensitive dye.
Following illumination, the dye is activated and produces singlet oxygen that damages
components of endothelial cell membranes, with subsequent platelet aggregation and
thrombi formation, which eventually determines the interruption of local blood flow. This
approach, initially proposed by Rosenblum and El-Sabban in 1977, was later improved by …