Detection of differential proteomes of human β-cells during islet-like differentiation using iTRAQ labeling
J Jin, J Park, K Kim, Y Kang, SG Park… - Journal of proteome …, 2009 - ACS Publications
Journal of proteome research, 2009•ACS Publications
A human β-cell line, RNAKT-15, was recently established from human pancreatic islets,
whereby its differentiation into islet-like β-cells (islet-like RNAKT-15) increased its
expression of insulin 2-fold compared with RNAKT-15 cells. To characterize the
differentiation of RNAKT-15 cells into islet-like RNAKT-15, microarray and quantitative
proteomics were performed. Our analysis of differential proteomic and mRNA expression
has resulted in a greater understanding of the molecular functions that are involved in β-cell …
whereby its differentiation into islet-like β-cells (islet-like RNAKT-15) increased its
expression of insulin 2-fold compared with RNAKT-15 cells. To characterize the
differentiation of RNAKT-15 cells into islet-like RNAKT-15, microarray and quantitative
proteomics were performed. Our analysis of differential proteomic and mRNA expression
has resulted in a greater understanding of the molecular functions that are involved in β-cell …
A human β-cell line, RNAKT-15, was recently established from human pancreatic islets, whereby its differentiation into islet-like β-cells (islet-like RNAKT-15) increased its expression of insulin 2-fold compared with RNAKT-15 cells. To characterize the differentiation of RNAKT-15 cells into islet-like RNAKT-15, microarray and quantitative proteomics were performed. Our analysis of differential proteomic and mRNA expression has resulted in a greater understanding of the molecular functions that are involved in β-cell differentiation and insulin synthesis and release.
ACS Publications