A computerized method for determination of microvascular density

MJ Rieder, DM O'Drobinak, AS Greene - Microvascular research, 1995 - Elsevier
Currently accepted stereological methods for vascular density measurements involve
manual counting of labeled vessels with a grid image overlay and determination of vessel-
grid intersections. This method both is tedious and may be prone to error; therefore, our
laboratory has developed a method for computer-automated determination of microvascular
density using digital image processing techniques. An image of the microvasculature is
acquired using computer videomicroscopy. The image is processed in three general steps …