[HTML][HTML] Identification of Neuronal RNA Targets of TDP-43-containing Ribonucleoprotein Complexes*♦
TAR DNA-binding protein 43 (TDP-43) is associated with a spectrum of neurodegenerative
diseases. Although TDP-43 resembles heterogeneous nuclear ribonucleoproteins, its RNA
targets and physiological protein partners remain unknown. Here we identify RNA targets of
TDP-43 from cortical neurons by RNA immunoprecipitation followed by deep sequencing
(RIP-seq). The canonical TDP-43 binding site (TG) n is 55.1-fold enriched, and moreover, a
variant with adenine in the middle,(TG) n TA (TG) m, is highly abundant among reads in our …
diseases. Although TDP-43 resembles heterogeneous nuclear ribonucleoproteins, its RNA
targets and physiological protein partners remain unknown. Here we identify RNA targets of
TDP-43 from cortical neurons by RNA immunoprecipitation followed by deep sequencing
(RIP-seq). The canonical TDP-43 binding site (TG) n is 55.1-fold enriched, and moreover, a
variant with adenine in the middle,(TG) n TA (TG) m, is highly abundant among reads in our …