[HTML][HTML] Rho activation regulates CXCL12 chemokine stimulated actin rearrangement and restitution in model intestinal epithelia

RA Moyer, MK Wendt, PA Johanesen, JR Turner… - Laboratory …, 2007 - Elsevier
Chemokines are critical regulatory factors that direct migration, proliferation and maturation
of receptor expressing target cells within gut mucosa. The aim of the present study was to
define the cellular mechanisms whereby engagement of the essential chemokine CXCL12
to CXCR4 regulates restitutive epithelial cell migration. Non-transformed IEC-6 cells or
polarized T84 epithelial monolayers were wounded and F-actin accumulation assessed
using fluorescence microscopy and flow cytometry. Immunoblot analysis, pull-down assays …