Stem cell marker CD133 affects clinical outcome in glioma patients
F Zeppernick, R Ahmadi, B Campos, C Dictus… - Clinical cancer …, 2008 - AACR
F Zeppernick, R Ahmadi, B Campos, C Dictus, BM Helmke, N Becker, P Lichter, A Unterberg…
Clinical cancer research, 2008•AACRPurpose: The CD133 antigen has been identified as a putative stem cell marker in normal
and malignant brain tissues. In gliomas, it is used to enrich a subpopulation of highly
tumorigenic cancer cells. According to the cancer stem cell hypothesis, CD133-positive cells
determine long-term tumor growth and, therefore, are suspected to influence clinical
outcome. To date, a correlation between CD133 expression in primary tumor tissues and
patients' prognosis has not been reported. Experimental Design: To address this question …
and malignant brain tissues. In gliomas, it is used to enrich a subpopulation of highly
tumorigenic cancer cells. According to the cancer stem cell hypothesis, CD133-positive cells
determine long-term tumor growth and, therefore, are suspected to influence clinical
outcome. To date, a correlation between CD133 expression in primary tumor tissues and
patients' prognosis has not been reported. Experimental Design: To address this question …
Abstract
Purpose: The CD133 antigen has been identified as a putative stem cell marker in normal and malignant brain tissues. In gliomas, it is used to enrich a subpopulation of highly tumorigenic cancer cells. According to the cancer stem cell hypothesis, CD133-positive cells determine long-term tumor growth and, therefore, are suspected to influence clinical outcome. To date, a correlation between CD133 expression in primary tumor tissues and patients' prognosis has not been reported.
Experimental Design: To address this question, we analyzed the expression of the CD133 stem cell antigen in a series of 95 gliomas of various grade and histology by immunohistochemistry on cryostat sections. Staining data were correlated with patient outcome.
Results: By multivariate survival analysis, we found that both the proportion of CD133-positive cells and their topological organization in clusters were significant (P < 0.001) prognostic factors for adverse progression-free survival and overall survival independent of tumor grade, extent of resection, or patient age. Furthermore, proportion of CD133-positive cells was an independent risk factor for tumor regrowth and time to malignant progression in WHO grade 2 and 3 tumors.
Conclusions: These findings constitute the first conclusive evidence that CD133 stem cell antigen expression correlates with patient survival in gliomas, lending support to the current cancer stem cell hypothesis.
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