Regulation of Clara cell secretory protein gene transcription by thyroid transcription factor-1
L Zhang, JA Whitsett, BR Stripp - … Et Biophysica Acta (BBA)-Gene Structure …, 1997 - Elsevier
CCSP is a 16 kDa protein expressed selectively in the Clara cells of the lung. Cis-acting
elements conferring Clara cell-specific expression of rat CCSP gene were contained within
the sequences− 320 to+ 57 of the rCCSP promoter. DNA-TTF-1 protein interactions were
identified within the sequences− 302 to− 278 and− 90 to− 66 from the transcriptional start
site of the rat CCSP gene promoter. In electrophoretic mobility shift assays, recombinant TTF-
1 homeodomain protein bound to each site. Nuclear extracts from H441 adenocarcinoma …
elements conferring Clara cell-specific expression of rat CCSP gene were contained within
the sequences− 320 to+ 57 of the rCCSP promoter. DNA-TTF-1 protein interactions were
identified within the sequences− 302 to− 278 and− 90 to− 66 from the transcriptional start
site of the rat CCSP gene promoter. In electrophoretic mobility shift assays, recombinant TTF-
1 homeodomain protein bound to each site. Nuclear extracts from H441 adenocarcinoma …