Rapid elimination of mature autoreactive B cells demonstrated by Cre-induced change in B cell antigen receptor specificity in vivo
KP Lam, K Rajewsky - … of the National Academy of Sciences, 1998 - National Acad Sciences
KP Lam, K Rajewsky
Proceedings of the National Academy of Sciences, 1998•National Acad SciencesDeveloping autoreactive B cells edit their B cell antigen receptor (BCR) in the bone marrow
and are clonally deleted when they fail to reexpress an innocent BCR. Here, inducible Cre-
lox P-mediated gene inversion is used to change the specificity of the BCR on mature IgM+
IgD+ B cells in vivo to address the fate of lymphocytes encountering self-antigens at this
developmental stage. Expression of an autoreactive BCR on mature B cells leads to their
rapid elimination from the periphery, a process that is inhibited by constitutive bcl-2 …
and are clonally deleted when they fail to reexpress an innocent BCR. Here, inducible Cre-
lox P-mediated gene inversion is used to change the specificity of the BCR on mature IgM+
IgD+ B cells in vivo to address the fate of lymphocytes encountering self-antigens at this
developmental stage. Expression of an autoreactive BCR on mature B cells leads to their
rapid elimination from the periphery, a process that is inhibited by constitutive bcl-2 …
Developing autoreactive B cells edit their B cell antigen receptor (BCR) in the bone marrow and are clonally deleted when they fail to reexpress an innocent BCR. Here, inducible Cre-loxP-mediated gene inversion is used to change the specificity of the BCR on mature IgM+ IgD+ B cells in vivo to address the fate of lymphocytes encountering self-antigens at this developmental stage. Expression of an autoreactive BCR on mature B cells leads to their rapid elimination from the periphery, a process that is inhibited by constitutive bcl-2 transgene expression in an antigen dose-dependent manner. Thus, selection of mature B cells into the long-lived peripheral pool does not prevent their deletion upon encounter of self-antigens.
National Acad Sciences