Hepatitis B Virus-Infected HepG2hNTCP Cells Serve as a Novel Immunological Tool To Analyze the Antiviral Efficacy of CD8+ T Cells In Vitro

A Hoh, M Heeg, Y Ni, A Schuch, B Binder… - Journal of …, 2015 - Am Soc Microbiol
A Hoh, M Heeg, Y Ni, A Schuch, B Binder, N Hennecke, HE Blum, M Nassal, U Protzer
Journal of virology, 2015Am Soc Microbiol
ABSTRACT CD8+ T cells are the main effector lymphocytes in the control of hepatitis B virus
(HBV) infection. However, limitations of model systems, such as low infection rates, restrict
mechanistic studies of HBV-specific CD8+ T cells. Here, we established a novel
immunological cell culture model based on HBV-infected HepG2hNTCP cells that
endogenously processed viral antigens and presented them to HBV-specific CD8+ T cells.
This induced cytolytic and noncytolytic CD8+ T-cell effector functions and reduction of viral …
Abstract
CD8+ T cells are the main effector lymphocytes in the control of hepatitis B virus (HBV) infection. However, limitations of model systems, such as low infection rates, restrict mechanistic studies of HBV-specific CD8+ T cells. Here, we established a novel immunological cell culture model based on HBV-infected HepG2hNTCP cells that endogenously processed viral antigens and presented them to HBV-specific CD8+ T cells. This induced cytolytic and noncytolytic CD8+ T-cell effector functions and reduction of viral loads.
American Society for Microbiology