Toll-like receptor 4 mediates the early inflammatory response after cold ischemia/reperfusion
DJ Kaczorowski, A Nakao, KP Mollen… - …, 2007 - journals.lww.com
DJ Kaczorowski, A Nakao, KP Mollen, R Vallabhaneni, R Sugimoto, J Kohmoto, K Tobita…
Transplantation, 2007•journals.lww.comBackground. Ischemia/reperfusion (I/R) injury leads to graft dysfunction and may contribute
to alloimmune responses posttransplantation. The molecular mechanisms of cold I/R injury
are only partially characterized but may involve toll-like receptor (TLR)-4 activation by
endogenous ligands. We tested the hypothesis that TLR4 mediates the early inflammatory
response in the setting of cold I/R in a murine cardiac transplant model. Methods. Syngeneic
heart transplants were performed in mutant mice deficient in TLR4 signaling (C3H/HeJ) and …
to alloimmune responses posttransplantation. The molecular mechanisms of cold I/R injury
are only partially characterized but may involve toll-like receptor (TLR)-4 activation by
endogenous ligands. We tested the hypothesis that TLR4 mediates the early inflammatory
response in the setting of cold I/R in a murine cardiac transplant model. Methods. Syngeneic
heart transplants were performed in mutant mice deficient in TLR4 signaling (C3H/HeJ) and …
Abstract
Background.
Ischemia/reperfusion (I/R) injury leads to graft dysfunction and may contribute to alloimmune responses posttransplantation. The molecular mechanisms of cold I/R injury are only partially characterized but may involve toll-like receptor (TLR)-4 activation by endogenous ligands. We tested the hypothesis that TLR4 mediates the early inflammatory response in the setting of cold I/R in a murine cardiac transplant model.
Methods.
Syngeneic heart transplants were performed in mutant mice deficient in TLR4 signaling (C3H/HeJ) and wild-type mice (C3H/HeOuJ). Transplants were also performed between the strains (mutant hearts into wild-type recipients and the converse). Donor hearts were subjected to 2 hr of cold ischemia. The grafts were retrieved at 3 and 24 hr after reperfusion. Serum samples were collected for cytokine analysis. Reverse-transcription polymerase chain reaction and histologic analysis were used to assess intra-graft inflammation.
Results.
After transplant, serum tumor necrosis factor (TNF), interleukin (IL)-6, JE/monocyte chemotractant protein (MCP)-1, IL-1β, and troponin I levels, as well as intragraft TNF, IL-1β, IL-6, early growth response (EGR)-1, intercellular adhesion molecule (ICAM)-1, and inducible nitric oxide synthase (iNOS) mRNA levels, were significantly lower in the mutant→ mutant group compared to the wild-type→ wild-type group (P≤ 0.05). Intermediate levels of serum IL-6, JE/MCP-1, as well as intragraft TNF, IL-1β, IL-6, and ICAM-1 mRNA were observed after transplants in the mutant→ wild-type and wild-type→ mutant groups. Immunohistochemistry revealed less myocardial nuclear factor-κB nuclear translocation at and less neutrophil infiltration in the mutant→ mutant group compared to the wild-type→ wild-type group.
Conclusions.
These findings demonstrate that TLR4 signaling is central to both the systemic and intragraft inflammatory responses that occur after cold I/R in the setting of organ transplantation and that TLR4 signaling on both donor and recipient cells contributes to this response.
Lippincott Williams & Wilkins