[HTML][HTML] Hypertension-causing mutations in Cullin3 protein impair RhoA protein ubiquitination and augment the association with substrate adaptors
Cullin-Ring ubiquitin ligases regulate protein turnover by promoting the ubiquitination of
substrate proteins, targeting them for proteasomal degradation. It has been shown
previously that mutations in Cullin3 (Cul3) causing deletion of 57 amino acids encoded by
exon 9 (Cul3Δ9) cause hypertension. Moreover, RhoA activity contributes to vascular
constriction and hypertension. We show that ubiquitination and degradation of RhoA is
dependent on Cul3 in HEK293T cells in which Cul3 expression is ablated by either siRNA or …
substrate proteins, targeting them for proteasomal degradation. It has been shown
previously that mutations in Cullin3 (Cul3) causing deletion of 57 amino acids encoded by
exon 9 (Cul3Δ9) cause hypertension. Moreover, RhoA activity contributes to vascular
constriction and hypertension. We show that ubiquitination and degradation of RhoA is
dependent on Cul3 in HEK293T cells in which Cul3 expression is ablated by either siRNA or …