[HTML][HTML] Evidence against cellular internalization in vivo of NMO-IgG, aquaporin-4, and excitatory amino acid transporter 2 in neuromyelitis optica
J Ratelade, JL Bennett, AS Verkman - Journal of Biological Chemistry, 2011 - ASBMB
Autoantibodies against astrocyte water channel aquaporin-4 (AQP4) are thought to be
pathogenic in neuromyelitis optica (NMO). Prior work has suggested that a key component
of NMO autoantibody (NMO-IgG) pathogenesis is internalization of AQP4 and the associated
glutamate transporter EAAT2, leading to glutamate excitotoxicity. Here, we show selective
endocytosis of NMO-IgG and AQP4 in transfected cell cultures, but little internalization in
brain in vivo. AQP4-dependent endocytosis of NMO-IgG occurred rapidly in various AQP4 …
pathogenic in neuromyelitis optica (NMO). Prior work has suggested that a key component
of NMO autoantibody (NMO-IgG) pathogenesis is internalization of AQP4 and the associated
glutamate transporter EAAT2, leading to glutamate excitotoxicity. Here, we show selective
endocytosis of NMO-IgG and AQP4 in transfected cell cultures, but little internalization in
brain in vivo. AQP4-dependent endocytosis of NMO-IgG occurred rapidly in various AQP4 …