Mechanisms of acetylcholine receptor loss from the neuromuscular junction

AG Engel, G Fumagalli - Ciba Foundation Symposium 90 …, 1982 - Wiley Online Library
Ciba Foundation Symposium 90‐Receptors, Antibodies and Disease, 1982Wiley Online Library
(Method A, Table 1) end-plate receptors are labelled in vivo (as, for example, by labelling
AChRs in diaphragm by injecting 1251-a-bungarotoxin into the pleural cavity). After a fixed
interval, the labelled muscle is removed and the radioactivity remaining per end-plate
estimated. In the second method (Method B, Table 1) muscle strips labelled in vivo are
placed in organ culture and the release of radioactivity into the culture medium is monitored
in vitro. We recently devised a third method (Method C, Table 1). A selected limb muscle is …
(Method A, Table 1) end-plate receptors are labelled in vivo (as, for example, by labelling AChRs in diaphragm by injecting 1251-a-bungarotoxin into the pleural cavity). After a fixed interval, the labelled muscle is removed and the radioactivity remaining per end-plate estimated. In the second method (Method B, Table 1) muscle strips labelled in vivo are placed in organ culture and the release of radioactivity into the culture medium is monitored in vitro. We recently devised a third method (Method C, Table 1). A selected limb muscle is labelled with an intramuscular injection of a suitable dose of 1251-a-bungarotoxin in vivo and the loss of radioactivity from that muscle is then monitored daily, or more frequently, with an externally placed y-counter of fixed geometry (Fig. 3). This method determines AChR half-life in the living animal, allows close evaluation of experimental modifications of AChR
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