[HTML][HTML] Iron overload and gene expression in HepG2 cells: analysis by differential display

D Barisani, R Meneveri, E Ginelli, C Cassani, D Conte - Febs Letters, 2000 - Elsevier
D Barisani, R Meneveri, E Ginelli, C Cassani, D Conte
Febs Letters, 2000Elsevier
The aim of the present study was to evaluate the effect of iron overload on gene expression
in HepG2 cells by differential display. Iron-treated cells showed a 50% decrease in
apolipoprotein B100 (Apo B100) and a 2-and 3-fold increase in semaphorin cd100 and
aldose reductase mRNA, respectively, with parallel variations in Apo B100 and aldose
reductase proteins. These effects were time-dependent. Vitamin E prevented the increase in
aldose reductase expression, but had no effect on Apo B100 and semaphorin cd100 …
The aim of the present study was to evaluate the effect of iron overload on gene expression in HepG2 cells by differential display. Iron-treated cells showed a 50% decrease in apolipoprotein B100 (Apo B100) and a 2- and 3-fold increase in semaphorin cd100 and aldose reductase mRNA, respectively, with parallel variations in Apo B100 and aldose reductase proteins. These effects were time-dependent. Vitamin E prevented the increase in aldose reductase expression, but had no effect on Apo B100 and semaphorin cd100. Treatment with hydrogen peroxide and 4-hydroxy-2,3-nonenal increased only aldose reductase mRNA. These data suggest that iron can affect mRNA levels by lipid peroxidation-dependent and -independent pathways.
Elsevier