T cell repertoire following kidney transplantation revealed by high-throughput sequencing
L Lai, L Wang, H Chen, J Zhang, Q Yan, M Ou… - Transplant …, 2016 - Elsevier
Delayed T cell recovery and restricted T cell receptor (TCR) diversity after kidney
transplantation are associated with increased risks of infection and malignancy. Technical
challenges limit the faithful measurement of TCR diversity after kidney transplantation. In this
study, we used a combination of multiplex-PCR, Illumina sequencing and IMGT/HighV-
QUEST to directly assess millions of TCRs per individual before and at two time points after
kidney transplantation (1 days and 7 days after transplantation) in a cohort of 10 patients …
transplantation are associated with increased risks of infection and malignancy. Technical
challenges limit the faithful measurement of TCR diversity after kidney transplantation. In this
study, we used a combination of multiplex-PCR, Illumina sequencing and IMGT/HighV-
QUEST to directly assess millions of TCRs per individual before and at two time points after
kidney transplantation (1 days and 7 days after transplantation) in a cohort of 10 patients …