Angiogenic sprouting and capillary lumen formation modeled by human umbilical vein endothelial cells (HUVEC) in fibrin gels: the role of fibroblasts and Angiopoietin …
MN Nakatsu, RCA Sainson, JN Aoto, KL Taylor… - Microvascular …, 2003 - Elsevier
Angiogenesis is a multistep process of critical importance both in development and in
physiological and pathophysiological processes in the adult. It involves endothelial cell (EC)
sprouting from the parent vessel, followed by migration, proliferation, alignment, tube
formation, and anastomosis to other vessels. Several in vitro models have attempted to
recreate this complex sequence of events with varying degrees of success. We report an
optimized protocol for human umbilical vein EC in which EC sprout from the surface of …
physiological and pathophysiological processes in the adult. It involves endothelial cell (EC)
sprouting from the parent vessel, followed by migration, proliferation, alignment, tube
formation, and anastomosis to other vessels. Several in vitro models have attempted to
recreate this complex sequence of events with varying degrees of success. We report an
optimized protocol for human umbilical vein EC in which EC sprout from the surface of …