Improved flow-based method for HIV/SIV envelope-specific memory B-cell evaluation in rhesus macaques
V Mohanram, T Demberg, I Tuero… - Journal of …, 2014 - Elsevier
The ability to elicit potent and long-lasting broadly neutralizing HIV envelope (Env)-specific
antibodies has become a key goal for HIV vaccine development. Consequently, the ability to
rapidly and efficiently monitor development of memory B cells in pre-clinical and clinical
vaccine trails is critical for continued progress in vaccine design. We have developed an
improved flow cytometry-based method for the rapid and efficient identification of gp120-
specific memory B cells in peripheral blood, bone marrow, and mucosal tissues which …
antibodies has become a key goal for HIV vaccine development. Consequently, the ability to
rapidly and efficiently monitor development of memory B cells in pre-clinical and clinical
vaccine trails is critical for continued progress in vaccine design. We have developed an
improved flow cytometry-based method for the rapid and efficient identification of gp120-
specific memory B cells in peripheral blood, bone marrow, and mucosal tissues which …