Rapid isolation and characterization of native mouse complement components C3 and C5

CW Van den Berg, H Van Dijk, PJA Capel - Journal of immunological …, 1989 - Elsevier
A rapid, 1 day procedure for the purification of mouse complement factors C3 and C5 is
described. The method is based on fractionated precipitation by polyethylene glycol 6000,
followed by Mono Q anion exchange chromatography on a system for fast protein liquid
chromatography (FPLC). For C3 isolation, an additional FPLC separation step using
Superose 12 (gel filtration) was used. C3 was purified 71-fold with a yield of 32% as
measured by biological activity; the preparation contained no detectable contaminants as …