[HTML][HTML] Potential Role for HIV-Specific CD38−/HLA-DR+ CD8+ T Cells in Viral Suppression and Cytotoxicity in HIV Controllers
S Hua, C Lecuroux, A Saez-Cirion, G Pancino, I Girault… - PloS one, 2014 - journals.plos.org
S Hua, C Lecuroux, A Saez-Cirion, G Pancino, I Girault, P Versmisse, F Boufassa, O Taulera…
PloS one, 2014•journals.plos.orgBackground HIV controllers (HIC) are rare HIV-1-infected patients who exhibit spontaneous
viral control. HIC have high frequency of CD38−/HLA-DR+ HIV-specific CD8+ T cells. Here
we examined the role of this subset in HIC status. Materials and Methods We compared
CD38−/HLA-DR+ CD8+ T cells with the classical CD38+/HLA-DR+ activated phenotype in
terms of 1) their activation status, reflected by CD69, CD25, CD71, CD40 and Ki67
expression, 2) functional parameters: Bcl-2 expression, proliferative capacity, and IFN-γ and …
viral control. HIC have high frequency of CD38−/HLA-DR+ HIV-specific CD8+ T cells. Here
we examined the role of this subset in HIC status. Materials and Methods We compared
CD38−/HLA-DR+ CD8+ T cells with the classical CD38+/HLA-DR+ activated phenotype in
terms of 1) their activation status, reflected by CD69, CD25, CD71, CD40 and Ki67
expression, 2) functional parameters: Bcl-2 expression, proliferative capacity, and IFN-γ and …
Background
HIV controllers (HIC) are rare HIV-1-infected patients who exhibit spontaneous viral control. HIC have high frequency of CD38−/HLA-DR+ HIV-specific CD8+ T cells. Here we examined the role of this subset in HIC status.
Materials and Methods
We compared CD38−/HLA-DR+ CD8+ T cells with the classical CD38+/HLA-DR+ activated phenotype in terms of 1) their activation status, reflected by CD69, CD25, CD71, CD40 and Ki67 expression, 2) functional parameters: Bcl-2 expression, proliferative capacity, and IFN-γ and IL-2 production, and 3) cytotoxic activity. We also investigated how this particular profile is generated.
Results
Compared to CD38+/HLA-DR+ cells, CD38−/HLA-DR+ cells exhibited lower expression of several activation markers, better survival capacity (Bcl-2 MFI, 367 [134–462] vs 638 [307–747], P = 0.001), higher frequency of polyfunctional cells (15% [7%–33%] vs 21% [16%–43%], P = 0.0003), greater proliferative capacity (0-fold [0–2] vs 3-fold –, P = 0.007), and higher cytotoxicity in vitro (7% [3%–11%] vs 13% [6%–22%], P = 0.02). The CD38−/HLA-DR+ profile was preferentially generated in response to low viral antigen concentrations.
Conclusions
These data highlight the role of CD38−/HLA-DR+ HIV-specific CD8+ T cell cytotoxicity in HIC status and provide insights into the mechanism by which they are generated. Induction of this protective CD8+ subset may be important for vaccine strategies.
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