Method for measurement of peroxisomal very long-chain fatty acid beta-oxidation and de novo C26: 0 synthesis activity in living cells using stable-isotope labeled …

MC van de Beek, IME Dijkstra, S Kemp - Peroxisomes: Methods and …, 2017 - Springer
MC van de Beek, IME Dijkstra, S Kemp
Peroxisomes: Methods and Protocols, 2017Springer
Peroxisomes are present in virtually every eukaryotic cell type with the exception of the
mature erythrocyte. In higher eukaryotes, one of the main functions of peroxisomes is lipid
metabolism by means of beta-oxidation of very long-chain fatty acids (VLCFA;≥ 22 carbon
atoms). A dysfunction in peroxisomal VLCFA beta-oxidation results in elevated VLCFA
levels in cells, tissue, and plasma. Here, we describe a straightforward and sensitive method
to measure peroxisomal beta-oxidation capacity in living cells using stable-isotope labeled …
Abstract
Peroxisomes are present in virtually every eukaryotic cell type with the exception of the mature erythrocyte. In higher eukaryotes, one of the main functions of peroxisomes is lipid metabolism by means of beta-oxidation of very long-chain fatty acids (VLCFA; ≥22 carbon atoms). A dysfunction in peroxisomal VLCFA beta-oxidation results in elevated VLCFA levels in cells, tissue, and plasma. Here, we describe a straightforward and sensitive method to measure peroxisomal beta-oxidation capacity in living cells using stable-isotope labeled docosanoic acid (D3-C22:0).
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