[HTML][HTML] Adeno-associated virus vector integration

DR Deyle, DW Russell - Current opinion in molecular therapeutics, 2009 - ncbi.nlm.nih.gov
DR Deyle, DW Russell
Current opinion in molecular therapeutics, 2009ncbi.nlm.nih.gov
Adeno-associated virus (AAV) vectors efficiently transduce various cell types and can
produce long-term expression of transgenes in vivo. Although AAV vector genomes can
persist within cells as episomes, vector integration has been observed in various
experimental settings, either at non-homologous sites where DNA damage may have
occurred or by homologous recombination. In some cases, integration is essential for the
therapeutic or experimental efficacy of AAV vectors. Recently, insertional mutagenesis …
Abstract
Adeno-associated virus (AAV) vectors efficiently transduce various cell types and can produce long-term expression of transgenes in vivo. Although AAV vector genomes can persist within cells as episomes, vector integration has been observed in various experimental settings, either at non-homologous sites where DNA damage may have occurred or by homologous recombination. In some cases, integration is essential for the therapeutic or experimental efficacy of AAV vectors. Recently, insertional mutagenesis resulting from the integration of AAV vectors was associated with tumorigenesis in mice, a consideration that may have relevance for certain clinical applications.
ncbi.nlm.nih.gov