DNA repair capacity measured by high throughput alkaline comet assays in EBV-transformed cell lines and peripheral blood cells from cancer patients and healthy …
M Iwakawa, M Goto, S Noda, M Sagara… - … /Genetic Toxicology and …, 2005 - Elsevier
We collected peripheral blood (PB) from 556 patients with various types of cancer who had
undergone radiotherapy and from 81 healthy volunteers. We exposed whole PB and Epstein-
Barr virus-transformed lymphoblastoid cell lines (EBLs) derived from the PB
mononucleocytes to X-irradiation (5Gy). Using the alkaline comet assay, we measured the
immediate DNA damage and, at 15min, the% residual damage. In PB, the immediate
damage was similar in patients and healthy volunteers while the% residual damage …
undergone radiotherapy and from 81 healthy volunteers. We exposed whole PB and Epstein-
Barr virus-transformed lymphoblastoid cell lines (EBLs) derived from the PB
mononucleocytes to X-irradiation (5Gy). Using the alkaline comet assay, we measured the
immediate DNA damage and, at 15min, the% residual damage. In PB, the immediate
damage was similar in patients and healthy volunteers while the% residual damage …