The voltage-dependent anion channel (VDAC), located at the outer mitochondria membrane (OMM), mediates interactions between mitochondria and other parts of the cell by transporting anions, cations, ATP, Ca²⁺, and metabolites. Substantial evidence points to VDAC1 as being a key player in apoptosis, regulating the release of apoptogenic proteins from mitochondria, such as cytochrome c, and interacting with anti-apoptotic proteins. Recently, we demonstrated that VDAC1 oligomerization is a general mechanism common to numerous apoptogens acting via different initiating cascades and proposed that a protein-conducting channel formed within a VDAC1 homo/hetero oligomer mediates cytochrome c release. However, the molecular mechanism responsible for VDAC1 oligomerization remains unclear. Several studies have shown that mitochondrial Ca²⁺ is involved in apoptosis induction and that VDAC1 possesses Ca²⁺-binding sites and mediates Ca²⁺ transport across the OMM. Here, the relationship between the cellular Ca²⁺ level, [Ca²⁺]_i, VDAC1 oligomerization and apoptosis was studied. Decreasing [Ca²⁺]_i using the cell-permeable Ca²⁺ chelating reagent BAPTA-AM was found to inhibit VDAC1 oligomerization and apoptosis, while increasing [Ca²⁺]_i using Ca²⁺ ionophore resulted in VDAC1 oligomerization and apoptosis induction in the absence of apoptotic stimuli. Moreover, induction of apoptosis elevated [Ca²⁺]_i, concomitantly with VDAC1 oligomerization. AzRu-mediated inhibition of mitochondrial Ca²⁺ transport decreased VDAC1 oligomerization, suggesting that mitochondrial Ca²⁺ is required for VDAC1 oligomerization. In addition, increased [Ca²⁺]_i levels up-regulate VDAC1 expression. These results suggest that Ca²⁺ promotes VDAC1 oligomerization via activation of a yet unknown signaling pathway or by increasing VDAC1 expression, leading to apoptosis. This article is part of a Special Issue entitled: 12th European Symposium on Calcium.