[HTML][HTML] Assembly and secretion of chylomicrons by differentiated Caco-2 cells: nascent triglycerides and preformed phospholipids are preferentially used for …
J Luchoomun, MM Hussain - Journal of Biological Chemistry, 1999 - ASBMB
To develop a cell culture model for chyclomicron (CM) assembly, the apical media of
differentiated Caco-2 cells were supplemented with oleic acid (OA) together with either
albumin or taurocholate (TC). The basolateral media were subjected to sequential density
gradient ultracentrifugations to obtain large CM, small CM, and very low density lipoproteins
(VLDL), and the distribution of apoB in these fractions was quantified. In the absence of OA,
apoB was secreted as VLDL/LDL size particles. Addition of OA (≥ 0.8 mm) with TC, but not …
differentiated Caco-2 cells were supplemented with oleic acid (OA) together with either
albumin or taurocholate (TC). The basolateral media were subjected to sequential density
gradient ultracentrifugations to obtain large CM, small CM, and very low density lipoproteins
(VLDL), and the distribution of apoB in these fractions was quantified. In the absence of OA,
apoB was secreted as VLDL/LDL size particles. Addition of OA (≥ 0.8 mm) with TC, but not …