[HTML][HTML] Target selectivity of vertebrate notch proteins: Collaboration between discrete domains and CSL-binding site architecture determines activation probability
CT Ong, HT Cheng, LW Chang, T Ohtsuka… - Journal of Biological …, 2006 - ASBMB
All four mammalian Notch proteins interact with a single DNA-binding protein (RBP-jκ), yet
they are not equivalent in activating target genes. Parallel assays of three Notch-responsive
promoters in several cell lines revealed that relative activation strength is dependent on
protein module and promoter context more than the cellular context. Each Notch protein
reads binding site orientation and distribution on the promoter differently; Notch1 performs
extremely well on paired sites, and Notch3 prefers single sites in conjunction with a proximal …
they are not equivalent in activating target genes. Parallel assays of three Notch-responsive
promoters in several cell lines revealed that relative activation strength is dependent on
protein module and promoter context more than the cellular context. Each Notch protein
reads binding site orientation and distribution on the promoter differently; Notch1 performs
extremely well on paired sites, and Notch3 prefers single sites in conjunction with a proximal …