Use and properties of ROCK-specific inhibitor Y-27632

S Narumiya, T Ishizaki, M Ufhata - Methods in enzymology, 2000 - Elsevier
Publisher Summary This chapter discusses the use and properties of Rho kinase (ROCK)-
specific inhibitor Y-27632. The chapter describes three examples of in vitro applications of Y-
27632 in tissues and cultured cells. Y-27632 and related compounds specifically bind to the
ROCK family of kinases and inhibit their kinase activity. ROCK family of kinases is involved
in the increase of actomyosin-based contractility through phosphorylation and down
regulation of myosin phosphatase. Y-27632 inhibits ROCK by competing with ATP for …