Hemoglobin is expressed in alveolar epithelial type II cells

M Bhaskaran, H Chen, Z Chen, L Liu - Biochemical and biophysical …, 2005 - Elsevier
M Bhaskaran, H Chen, Z Chen, L Liu
Biochemical and biophysical research communications, 2005Elsevier
Hemoglobin is the main oxygen carrying heme protein in erythrocytes. In an effort to study
the differential gene expression of alveolar epithelial type I and type II cells using DNA
microarray technique, we found that the mRNAs of hemoglobin α-and β-chains were
expressed in type II cells, but not in type I cells. The microarray data were confirmed by RT-
PCR. The mRNA expression of both chains decreased when type II cells trans-differentiated
into type I-like cells. Immunocyto/histochemistry revealed that hemoglobin protein was …
Hemoglobin is the main oxygen carrying heme protein in erythrocytes. In an effort to study the differential gene expression of alveolar epithelial type I and type II cells using DNA microarray technique, we found that the mRNAs of hemoglobin α- and β-chains were expressed in type II cells, but not in type I cells. The microarray data were confirmed by RT-PCR. The mRNA expression of both chains decreased when type II cells trans-differentiated into type I-like cells. Immunocyto/histochemistry revealed that hemoglobin protein was specifically localized in type II cells of a lung cell mixture and rat lung tissue. The endogenous synthesis of hemoglobin in alveolar epithelial cells suggests that hemoglobin may have unidentified functions other than oxygen transport in the lung.
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