[HTML][HTML] Biochemical fates of α hemoglobin bound to α hemoglobin-stabilizing protein AHSP
Alpha hemoglobin-stabilizing protein (AHSP) is an erythroid protein that binds free α
hemoglobin (αHb) to maintain its structure and limit its pro-oxidant activity. Prior studies have
defined two different αHb· AHSP complexes. Binding of AHSP to Fe (II) αHb induces an
unusual configuration in which the F helix of the globin becomes disordered and the heme
ring becomes solvent-exposed. Over time, this intermediate oxidizes to form a stable
hemichrome in which the proximal (F8) and distal (E7) histidines are coordinated to the …
hemoglobin (αHb) to maintain its structure and limit its pro-oxidant activity. Prior studies have
defined two different αHb· AHSP complexes. Binding of AHSP to Fe (II) αHb induces an
unusual configuration in which the F helix of the globin becomes disordered and the heme
ring becomes solvent-exposed. Over time, this intermediate oxidizes to form a stable
hemichrome in which the proximal (F8) and distal (E7) histidines are coordinated to the …