A novel assay for apoptosis flow cytometric detection of phosphatidylserine expression on early apoptotic cells using fluorescein labelled annexin V

I Vermes, C Haanen, H Steffens-Nakken… - Journal of …, 1995 - Elsevier
In the early stages of apoptosis changes occur at the cell surface, which until now have
remained difficult to recognize. One of these plasma membrane alterations is the
translocation of phosphatidylserine (PS) from the inner side of the plasma membrane to the
outer layer, by which PS becomes exposed at the external surface of the cell. Annexin V is a
Ca2+ dependent phospholipid-binding protein with high affinity for PS. Hence this protein
can be used as a sensitive probe for PS exposure upon the cell membrane. Translocation of …