[HTML][HTML] Insights into hemoglobin assembly through in vivo mutagenesis of α-hemoglobin stabilizing protein
E Khandros, TL Mollan, X Yu, X Wang, Y Yao… - Journal of Biological …, 2012 - ASBMB
α-Hemoglobin stabilizing protein (AHSP) is believed to facilitate adult Hemoglobin A
assembly and protect against toxic free α-globin subunits. Recombinant AHSP binds
multiple forms of free α-globin to stabilize their structures and inhibit precipitation. However,
AHSP also stimulates autooxidation of αO 2 subunit and its rapid conversion to a partially
unfolded bishistidyl hemichrome structure. To investigate these biochemical properties, we
altered the evolutionarily conserved AHSP proline 30 in recombinantly expressed proteins …
assembly and protect against toxic free α-globin subunits. Recombinant AHSP binds
multiple forms of free α-globin to stabilize their structures and inhibit precipitation. However,
AHSP also stimulates autooxidation of αO 2 subunit and its rapid conversion to a partially
unfolded bishistidyl hemichrome structure. To investigate these biochemical properties, we
altered the evolutionarily conserved AHSP proline 30 in recombinantly expressed proteins …