[HTML][HTML] Prevention of CpG-Induced Pregnancy Disruption by Adoptive Transfer of In Vitro-Induced Regulatory T Cells
Objective To elucidate the mechanism by which embryo-resorption and preterm birth were
enhanced by pathogenic CpG motif and to develop a counter strategy for normal pregnancy
outcome. Methods This is an animal model-based study. In pregnant nonobese diabetic
(NOD) mice and wild-type (WT) mice in the same strain background, an infection was
mimicked by toll-like receptor 9 (TLR9) activation through CpG1826-injection. In vivo
inactivation of IL-10 was performed to enhance pregnancy loss. Regulatory T cells induced …
enhanced by pathogenic CpG motif and to develop a counter strategy for normal pregnancy
outcome. Methods This is an animal model-based study. In pregnant nonobese diabetic
(NOD) mice and wild-type (WT) mice in the same strain background, an infection was
mimicked by toll-like receptor 9 (TLR9) activation through CpG1826-injection. In vivo
inactivation of IL-10 was performed to enhance pregnancy loss. Regulatory T cells induced …
Objective
To elucidate the mechanism by which embryo-resorption and preterm birth were enhanced by pathogenic CpG motif and to develop a counter strategy for normal pregnancy outcome.
Methods
This is an animal model-based study. In pregnant nonobese diabetic (NOD) mice and wild-type (WT) mice in the same strain background, an infection was mimicked by toll-like receptor 9 (TLR9) activation through CpG1826-injection. In vivo inactivation of IL-10 was performed to enhance pregnancy loss. Regulatory T cells induced by FTY720 in vitro from splenic CD4+CD25−Foxp3− cells (iTreg cells) were transferred to improve pregnancy outcomes in NOD mice.
Results
Embryo-resorption and preterm birth were readily induced by CpG1826 in NOD mice, but not in WT mice. However, inactivation of IL-10 using neutralizing antibody injections enhanced pregnancy loss in WT mice exposed to CpG, while adoptive transfer of iTreg cells increased decidual Foxp3+ Treg cells and IL-10+ cell number and rescued pregnancy.
Conclusions
NOD mice are prone to abortion and preterm birth. This can be attributed to lacking Treg cells and insufficient IL-10 expression. Adoptive transfer of iTreg cells can rescue CpG-mediated pregnancy failure.
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