Optimization of storage and shipment of cryopreserved peripheral blood mononuclear cells from HIV-infected and uninfected individuals for ELISPOT assays
A Weinberg, LY Song, CL Wilkening, T Fenton… - Journal of …, 2010 - Elsevier
Functional immunologic assays using cryopreserved peripheral blood mononuclear cells
(PBMC) are influenced by blood processing, storage and shipment. The objective of this
study was to compare the viability, recovery and ELISPOT results of PBMC stored and
shipped in liquid nitrogen (LN/LN) or stored in LN and shipped on dry ice (LN/DI) or stored
at− 70° C for 3 to 12weeks and shipped on DI (70/DI 3 to 12); and to assess the effect of
donor HIV infection status on the interaction between storage/shipment and the outcome …
(PBMC) are influenced by blood processing, storage and shipment. The objective of this
study was to compare the viability, recovery and ELISPOT results of PBMC stored and
shipped in liquid nitrogen (LN/LN) or stored in LN and shipped on dry ice (LN/DI) or stored
at− 70° C for 3 to 12weeks and shipped on DI (70/DI 3 to 12); and to assess the effect of
donor HIV infection status on the interaction between storage/shipment and the outcome …